c peptide level  (Thermo Fisher)

Journal: Phytomedicine Plus

Article Title: The effects of glycoside-rich green extract from Centella asiatica (L.) Urban on wound healing and anti-aging activity

doi: 10.1016/j.phyplu.2024.100628

Figure Lengend Snippet: Fig. 5. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with glycoside-rich C. asiatica extracts (GRCs) at various concentrations (0.1, 1, and 10 µg/ml). *p < 0.05 vs. 1 % DMSO (control).

Article Snippet: After 24 hr of incubation, the culture supernatants were collected, and the PIP levels were determined by following the PIP ELISA Kit protocol (MK101, Takara Bio, Inc.).

Techniques: Control

Journal: Phytomedicine Plus

Article Title: The effects of glycoside-rich green extract from Centella asiatica (L.) Urban on wound healing and anti-aging activity

doi: 10.1016/j.phyplu.2024.100628

Figure Lengend Snippet: Fig. 6. Procollagen type I C-peptide (PIP) levels in HDF culture medium supernatants 24 hr after treatment with 10 µg/ml GRC1 and different components: total triterpenoids (MS+AS+MA+AA), glycosides (MS+AS), aglycones (MA+AA) and individual triterpenoids (MS, AS, MA, AA). *p < 0.05 vs. 1 % DMSO (control) #p < 0.05 vs. 10 µg/ml GRC1 MS=Madecassoside; AS=Asiaticoside; MA=Madecassic acid; AA=Asiatic acid.

Article Snippet: After 24 hr of incubation, the culture supernatants were collected, and the PIP levels were determined by following the PIP ELISA Kit protocol (MK101, Takara Bio, Inc.).

Techniques: Control

Journal: bioRxiv

Article Title: BLAST: A blue light-assisted secretion toolkit tunable by reversible protein-protein interactions

doi: 10.64898/2026.03.30.715452

Figure Lengend Snippet: Secretion of therapeutic proteins with a-BLAST and d-BLAST. (a) The human preproinsulin (preproINS) construct contains a signal peptide, B-chain (yellow), C-peptide (dark gray), and A-chain (yellow), with engineered Furin cleavage sites flanking the C-peptide for maturation. To facilitate efficient processing within the Golgi apparatus, Furin protease was co-transfected with the BLAST modules. (b) Kinetic profiling of light-induced insulin secretion. Summary graphs of secreted C-peptide levels, quantified by ELISA, as a proxy for insulin secretion from a-BLAST (left) and d-BLAST (right). Both systems exhibited significant, time-dependent insulin release starting from 2 h of illumination (8.2-fold for a-BLAST, 8.4-fold for d-BLAST), reaching maximal induction at 24 h (13.8-fold for a-BLAST, 19.3-fold for d-BLAST). (c) Plasmid configurations for IL-12 secretion. Schematic of the heterodimeric cytokine IL-12-a-BLAST (left) and d-BLAST-IL-12 (right) constructs. (d) Kinetic profiling of light-induced IL-12 secretion. Summary graphs showing IL-12 secretion levels measured by ELISA. Significant secretion was observed starting at 3 h for a-BLAST (2.5-fold) and 2 h for d-BLAST (2.2-fold). At the 24 h time point, d-BLAST (4.7-fold) demonstrated a slightly higher dynamic range compared to a-BLAST (4.5-fold). Open circles represent individual measurements from three biologically independent samples. Data are presented as means ± S.D. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparisons test (ns = not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Secreted human C-peptide levels in the supernatant were quantified using a Human C-peptide ELISA Kit (R&D Systems; Catalog #DICP00).

Techniques: Construct, Transfection, Enzyme-linked Immunosorbent Assay, Plasmid Preparation

Journal: BMC Medicine

Article Title: A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression

doi: 10.1186/s12916-024-03583-w

Figure Lengend Snippet: The relationship between the variant rs912304 and residual islet function and islet autoimmunity in T1D individuals. A - C The association between rs912304 and fasting C-peptide level in total newly diagnosed T1D subjects, those diagnosed age <12 and ≥12 years old, respectively. D - F The association between rs912304 and 2-hour C-peptide AUC in total newly diagnosed T1D subjects, those diagnosed age <12 and ≥12 years old, respectively. G - I The association between rs912304 and the positive rate of ZnT8A, GADA, and IA-2A in total T1D subjects. The genotype distribution of rs912304 in newly diagnosed T1D subjects: GG = 52, TG = 102, TT = 48; T1D subjects with diagnosed age <12: GG = 10, TG = 26, TT = 8; and T1D subjects with diagnosed age ≥12: GG = 42, TG = 76, TT = 40. The genotype distribution of rs912304 in islet autoimmunity: GG=616, GT=1004, TT=420 for ZnT8A; GG=644, GT=1061, TT=443 for GADA; GG=639, GT=1059, TT=443 for IA-2A. The above correlations were analyzed by linear regression analysis and were corrected for sex and duration of T1D disease. Data are mean with 95%CI. * P <0.05 was considered as significant

Article Snippet: This test was not required for T1D patients with fasting C-peptide levels ≤ 25 pmol/L, but only the fasting C-peptide level was measured by chemiluminescence (Roche Diagnostics, Switzerland).

Techniques: Variant Assay